Find and report all restriction enzyme cut sites in DNA sequences.
Paste one or more FASTA sequences (max 100,000,000 characters).
Treat sequences as linear or circular molecules.
Paste one or more DNA sequences in FASTA format. The tool supports sequences up to 100 million characters and automatically removes non-DNA characters. Ideal for plasmids, genes, or genomic regions.
Choose "Linear" for PCR products, genes, or linear plasmids. Choose "Circular" for plasmids and vectors to detect restriction sites that span the origin. Circular mode checks for sites across the sequence junction.
The output lists all restriction enzymes that cut your sequence, showing the enzyme name, recognition pattern, and exact positions (1-indexed) of each cut site. Enzymes are grouped by number of cuts for easy identification of unique cutters.
Use this tool for quick plasmid verification, finding unique restriction sites for cloning, checking for unwanted restriction sites in inserts, or planning restriction mapping experiments. Single-cutters are particularly useful for linearizing plasmids.
This tool scans DNA sequences for recognition sites of common restriction enzymes and reports their locations. Unlike restriction digest which physically cuts the DNA, restriction summary just identifies where enzymes would cut.
Quick restriction mapping for plasmid verification, checking for enzyme sites in sequences, and planning cloning strategies.