Protein Stats

Calculate amino acid composition and statistical analysis of protein sequences.

Tool Configuration
Configure the parameters for Protein Stats

Paste one or more FASTA sequences (max 500,000,000 characters).

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Input Protein Sequences

Paste one or more protein sequences in FASTA format using standard one-letter amino acid codes. The tool accepts sequences up to 500 million characters and automatically removes invalid characters. You can analyze multiple proteins simultaneously.

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Understanding Amino Acid Categories

Results group amino acids by chemical properties: Aliphatic (G, A, V, L, I - nonpolar), Aromatic (F, W, Y - ring structures), Sulfur-containing (C, M), Basic (K, R, H - positive), Acidic (D, E - negative), Hydroxyl (S, T), and Amide (N, Q). These categories help predict protein behavior and stability.

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Interpreting Composition Statistics

The output shows both counts and percentages for each amino acid and category. High percentages of hydrophobic residues suggest membrane proteins, while high charged residue content indicates soluble proteins. Cysteine content hints at disulfide bond formation potential.

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Applications

Use this analysis for predicting protein solubility, identifying unusual amino acid bias, comparing homologous proteins across species, quality checking recombinant proteins, and understanding sequence-structure-function relationships in protein engineering projects.

Amino Acid Composition
Understanding protein composition and chemical properties.

What is Amino Acid Composition?

Amino acid composition is the percentage or count of each amino acid residue in a protein sequence. This information is useful for understanding protein properties, predicting protein behavior, and comparing sequences across different organisms.

Amino Acid Categories

Amino acids are grouped into functional categories based on their chemical properties:
  • Aliphatic: Glycine, Alanine, Valine, Leucine, Isoleucine (nonpolar, hydrophobic)
  • Aromatic: Phenylalanine, Tryptophan, Tyrosine (contains aromatic rings)
  • Sulphur-containing: Cysteine, Methionine (contain sulfur atoms)
  • Basic: Lysine, Arginine, Histidine (positively charged)
  • Acidic: Aspartic acid, Glutamic acid (negatively charged)
  • Hydroxyl group: Serine, Threonine (contain hydroxyl groups)

Applications

Amino acid composition analysis is used for:
  • • Predicting protein solubility and hydropathy
  • • Identifying protein characteristics
  • • Protein classification and comparison
  • • Quality assessment of recombinant proteins
  • • Understanding sequence-structure-function relationships