Calculate the GRAVY (Grand Average of hydropathy) value for protein sequences.
Paste one or more FASTA sequences (max 500,000,000 characters).
Paste one or more protein sequences in FASTA format. The tool uses the Kyte-Doolittle hydropathy scale to calculate the average hydropathy value for each sequence. Multiple sequences can be analyzed simultaneously for comparative studies.
Positive GRAVY values indicate hydrophobic proteins (likely membrane proteins or proteins with hydrophobic cores). Negative GRAVY values indicate hydrophilic proteins (typically soluble in aqueous environments). Values typically range from -2.0 (very hydrophilic) to +2.0 (very hydrophobic).
Hydropathy measures how water-loving (hydrophilic) or water-fearing (hydrophobic) a protein is. This property affects protein folding, cellular localization, and function. Membrane proteins have high GRAVY values, while enzymes in aqueous environments have low GRAVY values.
Use GRAVY analysis to predict protein subcellular localization, identify membrane proteins, assess protein solubility for recombinant expression, compare related proteins across species, and guide protein engineering for improved solubility or membrane integration.
GRAVY stands for Grand Average of hydropathY. It is a measure of the overall hydropathy of a protein sequence, calculated by summing the hydropathy values of all amino acids and dividing by the sequence length.
The hydropathy values are based on the Kyte-Doolittle scale, which assigns each amino acid a value reflecting its hydrophobic (positive values) or hydrophilic (negative values) character.
Positive GRAVY: Indicates a hydrophobic protein (likely membrane-bound or protein-protein interaction domains)
Negative GRAVY: Indicates a hydrophilic protein (likely soluble in aqueous solution)
Alanine (1.8), Arginine (-4.5), Asparagine (-3.5), Aspartic acid (-3.5), Cysteine (2.5), Glutamine (-3.5), Glutamic acid (-3.5), Glycine (-0.4), Histidine (-3.2), Isoleucine (4.5), Leucine (3.8), Lysine (-3.9), Methionine (1.9), Phenylalanine (2.8), Proline (-1.6), Serine (-0.8), Threonine (-0.7), Tryptophan (-0.9), Tyrosine (-1.3), Valine (4.2)