Identify minimal nucleotide substitutions that create a chosen restriction site while monitoring protein translations.
Paste a raw sequence or FASTA-formatted entries (limit 10,000,000 characters). Non-DNA symbols are stripped before analysis.
Select the recognition site you would like to introduce by mutation.
Controls the width of each reported block of sequence and translation.
Choose which translation frames to render alongside the DNA sequence.
Select the translation table used when computing protein sequences.
Paste one or more DNA sequences in FASTA or raw format. Non-DNA characters are automatically removed (limit 10 million characters).
Choose the restriction enzyme you want to introduce. The tool identifies locations where minimal mutations (1-2 bases) can create the recognition site.
Set bases per line for output formatting and select which reading frames to display. Choose the appropriate genetic code for translation display.
Results show proposed mutations with current and mutated sequences side-by-side, including translations to verify the impact on protein coding regions.
Candidate mutation sites are generated by introducing one or two degenerate bases into the recognition motif and keeping suggestions that alter the DNA without overlapping existing enzyme matches. Suggested segments favour the minimal number of changes necessary to form the requested site.
Translations are rendered alongside the DNA to highlight how each proposed mutation influences protein-coding frames. Use the reading frame selector to inspect specific codons or to focus on uppercase characters already denoting coding regions in your input.