Convert DNA sequences to reverse, complement, or reverse-complement
Paste raw sequence or one or more FASTA sequences. Input limit is 100,000,000 characters.
Choose the transformation to apply to the sequences.
Enter your DNA sequences in FASTA format or as raw sequences. The tool supports standard IUPAC DNA codes including ambiguity codes.
Choose whether to generate the reverse-complement, reverse only, or complement only. Reverse-complement is most common for primer design.
Click execute to transform your sequences. The tool preserves case and properly handles all IUPAC ambiguity codes (R, Y, S, W, K, M, etc.).
Save the transformed sequences for use in primer design, ORF analysis, or sequence alignment applications.
Work with the reverse-complement if a sequence contains an open reading frame (ORF) on the reverse strand.
Generate reverse-complement sequences for designing reverse primers in PCR applications.
Prepare sequences for alignment when comparing forward and reverse strands.
Identify palindromic sequences and restriction sites by comparing sequences with their reverse-complements.